PITPNC1 Suppress CD8⁺ T cell immune function and promote radioresistance in rectal cancer by modulating FASN/CD155.

ABSTRACT:

Background

Radioresistance is a primary factor contributing to the failure of rectal cancer treatment. Immune suppression plays a significant role in the development of radioresistance. We have investigated the potential role of phosphatidylinositol transfer protein cytoplasmic 1 (PITPNC1) in regulating immune suppression associated with radioresistance.

Methods

To elucidate the mechanisms by which PITPNC1 influences radioresistance, we established HT29, SW480, and MC38 radioresistant cell lines. The relationship between radioresistance and changes in the proportion of immune cells was verified through subcutaneous tumor models and flow cytometry. Changes in the expression levels of PITPNC1, FASN, and CD155 were determined using immunohistochemistry and western blotting tech

SUBMITTER: Liang J 

PROVIDER: S-EPMC10826121 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature