Identification of genomic loci regulating platelet plasminogen activator inhibitor-1 in mice.

ABSTRACT:

Background

Plasminogen activator inhibitor-1 (PAI-1, Serpine1) is an important circulating fibrinolysis inhibitor. PAI-1 exists in 2 pools, packaged within platelet α-granules and freely circulating in plasma. Elevated plasma PAI-1 levels are associated with cardiovascular disease. However, little is known about the regulation of platelet PAI-1 (pPAI-1).

Objectives

We investigated the genetic control of pPAI-1 levels in mice and humans.

Methods

We measured pPAI-1 antigen levels via enzyme-linked immunosorbent assay in platelets isolated from 10 inbred mouse strains, including LEWES/EiJ (LEWES) and C57BL/6J (B6). LEWES and B6 were crossed to produce the F1 generation, B6LEWESF1. B6LEWESF1 mice were intercrossed to produce B6LEWESF2 mice. These mice were subjected to ge

SUBMITTER: Siebert AE 

PROVIDER: S-EPMC10826891 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature