The mixing method used to formulate lipid nanoparticles affects mRNA delivery efficacy and organ tropism.

ABSTRACT: mRNA is a versatile drug molecule with therapeutic applications ranging from protein replacement therapies to in vivo gene engineering. mRNA delivery is often accomplished using lipid nanoparticles, which are formulated via mixing of aqueous and organic solutions. Although this has historically been accomplished by manual mixing for bench scale science, microfluidic mixing is required for scalable continuous manufacturing and batch to batch control. Currently, there is limited understanding on how the mixing process affects mRNA delivery efficacy, particularly in regard to tropism. To address this knowledge gap, we examined the influence of the type of mixing and microfluidic mixing parameters on the performance of lipid nanoparticles in mice. This was accomplished with a Design of Experim

SUBMITTER: Strelkova Petersen DM 

PROVIDER: S-EPMC10826902 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature