Dataset Information

ZmNRT1.1B (ZmNPF6.6) determines nitrogen use efficiency via regulation of nitrate transport and signalling in maize.

ABSTRACT: Nitrate (NO₃ ^- ) is crucial for optimal plant growth and development and often limits crop productivity under low availability. In comparison with model plant Arabidopsis, the molecular mechanisms underlying NO₃ ^- acquisition and utilization remain largely unclear in maize. In particular, only a few genes have been exploited to improve nitrogen use efficiency (NUE). Here, we demonstrated that NO₃ ^- -inducible ZmNRT1.1B (ZmNPF6.6) positively regulated NO₃ ^- -dependent growth and NUE in maize. We showed that the tandem duplicated proteoform ZmNRT1.1C is irrelevant to maize seedling growth under NO₃ ^- supply; however, the loss of function of ZmNRT1.1B significantly weakened plant growth under adequate NO₃ ^- supply under both hydroponic and field conditions. The ¹⁵ N-labelled NO₃ ^- absorption assay indicated that ZmNRT1.1B mediated the high-affinity NO₃ ^- -transport and root-to-shoot NO₃ ^- translocation. Transcriptome analysis further showed, upon NO₃ ^- supply, ZmNRT1.1B promotes cytoplasmic-to-nuclear shuttling of ZmNLP3.1 (ZmNLP8), which co-regulates the expression of genes involved in NO₃ ^- response, cytokinin biosynthesis and carbon metabolism. Remarkably, overexpression of ZmNRT1.1B in modern maize hybrids improved grain yield under N-limiting fields. Taken together, our study revealed a crucial role of ZmNRT1.1B in high-affinity NO₃ ^- transport and signalling and offers valuable genetic resource for breeding N use efficient high-yield cultivars.

SUBMITTER: Cao H

PROVIDER: S-EPMC10826987 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature

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ZmNRT1.1B (ZmNPF6.6) determines nitrogen use efficiency via regulation of nitrate transport and signalling in maize.

Cao Huairong H Liu Zhi Z Guo Jia J Jia Zhongtao Z Shi Yandong Y Kang Kai K Peng Wushuang W Wang Zhangkui Z Chen Limei L Neuhaeuser Benjamin B Wang Yong Y Liu Xiangguo X Hao Dongyun D Yuan Lixing L

Plant biotechnology journal 20231002 2

Nitrate (NO3 - ) is crucial for optimal plant growth and development and often limits crop productivity under low availability. In comparison with model plant Arabidopsis, the molecular mechanisms underlying NO3 - acquisition and utilization remain largely unclear in maize. In particular, only a few genes have been exploited to improve nitrogen use efficiency (NUE). Here, we demonstrated that NO3 - -inducible ZmNRT1.1B (ZmNPF6.6) ...[more]

PMID: 37786281

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Project description:Mulching and nitrogen are critical drivers of crop production for smallholders of the Loess Plateau in China. The purpose of this study was to investigate the effect of mulching and nitrogen fertilizer on the soil water content, soil nitrate-N content and vertical distribution in maize root-zone. The experiment was conducted over two consecutive years and used randomly assigned field plots with three replicates. The six treatments consisted of no fertilizer without plastic film (CK), plastic film mulching with no basal fertilizer and no top dressing (MN0), basal fertilizer with no top dressing and no mulching (BN1), plastic film mulching and basal fertilizer with no top dressing (MN1), basal fertilizer and top dressing with no mulching (BN2) and plastic film mulching with basal fertilizer and top dressing (MN2). In the top soil layers, the soil water content was a little high in the plastic film mulching than that without mulching. The mean soil water content from 0 to 40 cm without mulching were 3.35% lower than those measured in the corresponding mulching treatments in 31 days after sowing in 2012. The mulching treatment increased the soil nitrate-N content was observed in the 0-40-cm soil layers. The results indicate that high contents of soil nitrate-N were mainly distributed at 0-20-cm at 31 days after sowing in 2012, and the soil nitrate-N concentration in the MN2 treatment was 1.58 times higher than that did not receive fertilizer. The MN2 treatment greatly increased the soil nitrate-N content in the upper layer of soil (0-40-cm), and the mean soil nitrate-N content was increased nearly 50 mg kg-1 at 105 days after sowing compared with CK treatment in 2012. The soil nitrate-N leaching amount in MN1 treatment was 28.61% and 39.14% lower than BN1 treatment, and the mulch effect attained to 42.55% and 65.27% in MN2 lower than BN2 in both years. The yield increased with an increase in the basal fertilizer, top dressing and plastic film mulching, and the grain yield increase ranged from 31.41% to 83.61% in two consecutive years. The MN1 and MN2 treatment is recommended because it increased the grain yield and improved the fertilizer use efficiency, compared with the no-mulching treatment.

Project description:Sub-optimal nitrogen (N) conditions reduce maize yield due to a decrease in two sink components: kernel set and potential kernel weight. Both components are established during the lag phase, suggesting that they could compete for resources during this critical period. However, whether this competition occurs or whether different genotypic strategies exist to optimize photoassimilate use during the lag phase is not clear and requires further investigation. We have addressed this knowledge gap by conducting a nutrient solution culture experiment that allows abrupt changes in N level and light intensity during the lag phase. We investigated plant growth, dry matter partitioning, non-structural carbohydrate concentration, N concentration, and 15N distribution (applied 4 days before silking) in plant organs at the beginning and the end of the lag phase in two maize hybrids that differ in grain yield under N-limited conditions: one is a nitrogen-use-efficient (EFFI) genotype and the other is a control (GREEN) genotype that does not display high N use efficiency. We found that the two genotypes used different mechanisms to regulate kernel set. The GREEN genotype showed a reduction in kernel set associated with reduced dry matter allocation to the ear during the lag phase, indicating that the reduced kernel set under N-limited conditions was related to sink restrictions. This idea was supported by a negative correlation between kernel set and sucrose/total sugar ratios in the kernels, indicating that the capacity for sucrose cleavage might be a key factor defining kernel set in the GREEN genotype. By contrast, the kernel set of the EFFI genotype was not correlated with dry matter allocation to the ear or to a higher capacity for sucrose cleavage; rather, it showed a relationship with the different EFFI ear morphology with bigger kernels at the apex of the ear than in the GREEN genotype. The potential kernel weight was independent of carbohydrate availability but was related to the N flux per kernel in both genotypes. In conclusion, kernel set and potential kernel weight are regulated independently, suggesting the possibility of simultaneously increasing both sink components in maize.

Dataset Information

ZmNRT1.1B (ZmNPF6.6) determines nitrogen use efficiency via regulation of nitrate transport and signalling in maize.

Publications

ZmNRT1.1B (ZmNPF6.6) determines nitrogen use efficiency via regulation of nitrate transport and signalling in maize.

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