<i>De novo</i>-designed minibinders expand the synthetic biology sensing repertoire.

ABSTRACT: Synthetic and chimeric receptors capable of recognizing and responding to user-defined antigens have enabled "smart" therapeutics based on engineered cells. These cell engineering tools depend on antigen sensors which are most often derived from antibodies. Advances in the de novo design of proteins have enabled the design of protein binders with the potential to target epitopes with unique properties and faster production timelines compared to antibodies. Building upon our previous work combining a de novo-designed minibinder of the Spike protein of SARS-CoV-2 with the synthetic receptor synNotch (SARSNotch), we investigated whether minibinders can be readily adapted to a diversity of cell engineering tools. We show that the Spike minibinder LCB1 easily generalizes to a next

SUBMITTER: Weinberg ZY 

PROVIDER: S-EPMC10827046 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature