A glimpse into the expanded genome content of Vibrio cholerae through identification of genes present in environmental strains.

ABSTRACT: Vibrio cholerae has multiple survival strategies which are reflected both in its broad distribution in many aquatic environments and its high genotypic diversity. To obtain additional information regarding the content of the V. cholerae genome, suppression subtractive hybridization (SSH) was used to prepare libraries of DNA sequences from two southern California coastal isolates which are divergent or absent in the clinical strain V. cholerae O1 El Tor N16961. More than 1,400 subtracted clones were sequenced. This revealed the presence of novel sequences encoding functions related to cell surface structures, transport, metabolism, signal transduction, luminescence, mobile elements, stress resistance, and virulence. Flanking sequence information was determined for loci of interest, and the

SUBMITTER: Purdy A 

PROVIDER: S-EPMC1082809 | biostudies-literature | 2005 May

REPOSITORIES: biostudies-literature