Effects of wasp (<i>Vespa crabro</i>) nest extracts on virus replication of <i>Autographa californica</i> nuclear polyhedrosis virus on <i>Spodoptera frugiperda</i> cell culture.

ABSTRACT: The antiviral properties of the extracts of Vespa crabro nests collected from the Black Sea, Turkey have been investigated on Spodoptera frugiperda (Sf) cell cultures of Autographa californica multicapsid nuclear polyhedrosis virus (AcMNPV). The effect of nests on cell viability and cytotoxicity analysis and the antiviral assay was studied, and the cytopathic effects of the virus were detected. The nest's viral content was identified. The impact of nest extracts on the protein synthesis of the virus was investigated. Also interaction with pUC18 plasmid DNA was investigated, to analyse the protective effects of the Vespa crabro nest extract againist to hydroxyl radical-mediated DNA damage. 50 µg/ml concentration of ethanol, acetone, and petroleum ether extracts o

SUBMITTER: Caldas A 

PROVIDER: S-EPMC10828140 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature