Deconstructing heterogeneity of replicative senescence in human mesenchymal stem cells at single cell resolution.

ABSTRACT: Following prolonged cell division, mesenchymal stem cells enter replicative senescence, a state of permanent cell cycle arrest that constrains the use of this cell type in regenerative medicine applications and that in vivo substantially contributes to organismal ageing. Multiple cellular processes such as telomere dysfunction, DNA damage and oncogene activation are implicated in promoting replicative senescence, but whether mesenchymal stem cells enter different pre-senescent and senescent states has remained unclear. To address this knowledge gap, we subjected serially passaged human ESC-derived mesenchymal stem cells (esMSCs) to single cell profiling and single cell RNA-sequencing during their progressive entry into replicative senescence. We found that esMSC transitioned through newly

SUBMITTER: Taherian Fard A 

PROVIDER: S-EPMC10828319 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature