Exosome-mediated circGMPS facilitates the development of gastric cancer cells through miR-144-3p/PUM1.

ABSTRACT: In recent years, gastric cancer (GC) is still one of the major public health burdens in the world. It is reported that exosome circular RNA (circRNA) is involved in the GC progression. However, the function and potential mechanism of circGMPS in GC remains unclear and needs further exploration. In this study, we isolated and identified exosomes from serum by TEM, NTA analysis and Western blot. RNA expression was evaluated by qRT-PCR. Western blot was employed to examine protein expression. Cell proliferation was measured using CCK-8. Transwell assay was adopted to analyze cell migration and invasion. The relationship between genes was explored through bioinformatics analysis, dual-luciferase reporter gene assay and spearman correlation coefficient. We found that circGMPS was elevated in GC

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC10828494 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature