Exploring how sucrose-colloid selection improves the fertilizing ability of chicken sperm after cryopreservation with glycerol.

ABSTRACT: Currently, glycerol is the most effective cryoprotectant when combined with straw packaging for preserving chicken sperm. Glycerol, however, has toxic effects on sperm cells, which can reduce fertility when present in inseminated semen. Historically, the serial dilution (SD) method was developed to eliminate glycerol and mitigate its adverse effects. We have recently developed a new method for removing glycerol called sucrose-Percoll (SP), that can be performed at either 4°C (4°C-SP) or 20°C (20°C-SP). This SP protocol has been found to be simpler and faster to improve fertility compared to the traditional SD method. Nevertheless, the reasons for such effectiveness differences between glycerol removal procedures remained unclear and required more comprehensive understandings for future pro

SUBMITTER: Lin HH 

PROVIDER: S-EPMC10828597 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature