YAP promotes the healing of ischemic wounds by reducing ferroptosis in skin fibroblasts through inhibition of ferritinophagy.

ABSTRACT: The impaired healing of chronic wounds is often attributed to the ischemic and hypoxic microenvironment, leading to increased cell death. Ferroptosis, a novel form of cell death unveiled in recent years, could potentially be linked with the process of wound healing. In this study, we explored the significance and mechanism of ferroptosis in ischemic wounds. Using transmission electron microscopy, Western blot, flow cytometry, immunofluorescence, and glutathione (GSH) assay, we observed that the death of primary mouse skin fibroblasts induced by oxygen and glucose deprivation (OGD) was associated with ferroptosis. Specifically, we observed elevated intracellular Fe²⁺ and lipid peroxidation levels and decreased GSH levels in vitro, indicative of ferroptosis. Importantly, we found

SUBMITTER: Cao G 

PROVIDER: S-EPMC10828694 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature