EN1 promotes lung metastasis of salivary adenoid cystic carcinoma by regulating the PI3K-AKT pathway and epithelial-mesenchymal transition.

ABSTRACT:

Background

Engrailed homeobox 1 (EN1) is a candidate oncogene that is epigenetically modified in salivary adenoid cystic carcinoma (SACC). We investigated the expression of EN1 in SACC tissues and cells, EN1 promoter methylation, and the role of EN1 in tumour progression in SACC.

Methods

Thirty-five SACC samples were screened for key transcription factors that affect tumour progression. In vitro and in vivo assays were performed to determine the viability, tumorigenicity, and metastatic ability of SACC cells with modulated EN1 expression. Quantitative methylation-specific polymerase chain reaction analysis was performed on SACC samples.

Results

EN1 was identified as a transcription factor that was highly overexpressed in SACC tissues, regardless of clinical stage and

SUBMITTER: Cui Y 

PROVIDER: S-EPMC10829235 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature