CRISPR/Cas9 mediated Y-chromosome elimination affects human cells transcriptome.

ABSTRACT:

Background

Sexual dimorphism represents a key concept in the comprehension of molecular processes guiding several sex-specific physiological and pathological mechanisms. It has been reported that genes involved in many disorders show a sex-dependent expression pattern. Moreover, the loss of Y chromosome (LOY), found to be a physiological age-driven phenomenon, has been linked to many neurodegenerative and autoimmune disorders, and to an increased cancer risk. These findings drove us towards the consideration that LOY may cause the de-regulation of disease specific networks, involving genes located in both autosomal and sex chromosomes.

Results

Exploiting the CRISPR/Cas9 and RNA-sequencing technologies, we generated a Y-deficient human cell line that has been investigated for

SUBMITTER: Celli L 

PROVIDER: S-EPMC10829266 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature