Piezo Mediates the Mechanosensation and Injury-Repair of Pulpo-Dentinal Complex.

ABSTRACT:

Objectives

The aim of this research was to investigate the functions of Piezo channels in dentin defect, including mechanical signalling and odontoblast responses.

Methods

Rat dentin-defect models were constructed, and spatiotemporal expression of Piezo proteins was detected in the pulpo-dentinal complex. Real-time polymerase chain reaction (rtPCR) was used to investigate the functional expression pattern of Piezo channels in odontoblasts. Moreover, RNA interference technology was employed to uncover the underlying mechanisms of the Piezo-driven inflammatory response and repair under fluid shear stress (FSS) conditions in vitro.

Results

Piezo1 and Piezo2 were found to be widely expressed in the odontoblast layer and dental pulp in the rat dentin-defect model during th

SUBMITTER: Xu X 

PROVIDER: S-EPMC10829354 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature