Transport of metformin metabolites by guanidinium exporters of the small multidrug resistance family.

ABSTRACT: Proteins from the small multidrug resistance (SMR) family are frequently associated with horizontally transferred multidrug resistance gene arrays found in bacteria from wastewater and the human-adjacent biosphere. Recent studies suggest that a subset of SMR transporters might participate in the metabolism of the common pharmaceutical metformin by bacterial consortia. Here, we show that both genomic and plasmid-associated transporters of the SMRGdx functional subtype export byproducts of microbial metformin metabolism, with particularly high export efficiency for guanylurea. We use solid-supported membrane electrophysiology to evaluate the transport kinetics for guanylurea and native substrate guanidinium by four representative SMRGdx homologs. Using an internal reference to normalize inde

SUBMITTER: Lucero RM 

PROVIDER: S-EPMC10829512 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature