Nicotine destructs dental stem cell-based periodontal tissue regeneration.

ABSTRACT:

Background/purpose

Nicotine is a widely known addictive and toxic substance in cigarette that exacerbates periodontitis. However, its deleterious effects on dental stem cells and subsequent implications in tissue regeneration remain unclear. This study aimed to explore the effects of nicotine on the regenerative capacity of human periodontal ligament stem cells (hPDLSCs) based on transcriptomics and proteomics, and determined possible targeted genes associated with smoking-related periodontitis.

Materials and methods

hPDLSCs were treated with different concentrations of nicotine ranging from 10^-3 to 10^-8 M. Transcriptomics and proteomics were performed and confirmed employing Western blot, 5-ethynyl-2'-deoxyuridine (EdU), and alkaline phosphatase (ALP)

SUBMITTER: Jiang Y 

PROVIDER: S-EPMC10829564 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature