Cellular origin and molecular mechanisms of lung metastases in patients with aggressive hepatoblastoma.

ABSTRACT:

Background and aims

Lung metastases are the most threatening signs for patients with aggressive hepatoblastoma (HBL). Despite intensive studies, the cellular origin and molecular mechanisms of lung metastases in patients with aggressive HBL are not known. The aims of these studies were to identify metastasis-initiating cells in primary liver tumors and to determine if these cells are secreted in the blood, reach the lung, and form lung metastases.

Approach

We have examined mechanisms of activation of key oncogenes in primary liver tumors and lung metastases and the role of these mechanisms in the appearance of metastasis-initiating cells in patients with aggressive HBL by RNA-Seq, immunostaining, chromatin immunoprecipitation, Real-Time Quantitative Reverse Transcription PCR

SUBMITTER: Gulati R 

PROVIDER: S-EPMC10830083 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature