Motion of VAPB molecules reveals ER-mitochondria contact site subdomains.

ABSTRACT: To coordinate cellular physiology, eukaryotic cells rely on the rapid exchange of molecules at specialized organelle-organelle contact sites^1,2. Endoplasmic reticulum-mitochondrial contact sites (ERMCSs) are particularly vital communication hubs, playing key roles in the exchange of signalling molecules, lipids and metabolites^3,4. ERMCSs are maintained by interactions between complementary tethering molecules on the surface of each organelle^5,6. However, due to the extreme sensitivity of these membrane interfaces to experimental perturbation^7,8, a clear understanding of their nanoscale organization and regulation is still lacking. Here we combine three-dimensional electron microscopy with high-speed molecular tracking of a model organelle tether,

SUBMITTER: Obara CJ 

PROVIDER: S-EPMC10830423 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature