Impact of the hypoxic microenvironment on spermatogonial stem cells in culture.

ABSTRACT: The stem cell niche plays a crucial role in the decision to either self-renew or differentiate. Recent observations lead to the hypothesis that O₂ supply by blood and local O₂ tension could be key components of the testicular niche of spermatogonial stem cells (SSCs). In this study, we investigated the impact of different hypoxic conditions (3.5%, 1%, and 0.1% O₂ tension) on murine and human SSCs in culture. We observed a deleterious effect of severe hypoxia (1% O₂ and 0.1% O₂) on the capacity of murine SSCs to form germ cell clusters when plated at low density. Severe effects on SSCs proliferation occur at an O₂ tension ≤1% and hypoxia was shown to induce a slight differentiation bias under 1% and 0.1% O₂ conditi

SUBMITTER: Gille AS 

PROVIDER: S-EPMC10830753 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature