DNA demethylation in the hypothalamus promotes transcription of Agtr1a and Slc12a2 and hypertension development.

ABSTRACT: Increased expression of angiotensin II AT_1A receptor (encoded by Agtr1a) and Na⁺-K⁺-Cl^- cotransporter-1 (NKCC1, encoded by Slc12a2) in the hypothalamic paraventricular nucleus (PVN) contributes to hypertension development. However, little is known about their transcriptional control in the PVN in hypertension. DNA methylation is a critical epigenetic mechanism that regulates gene expression. Here, we determined whether transcriptional activation of Agtr1a and Slc12a2 results from altered DNA methylation in spontaneously hypertensive rats (SHR). Methylated DNA immunoprecipitation and bisulfite sequencing-PCR showed that CpG methylation at Agtr1a and Slc12a2 promoters in the PVN was progressively diminished in SHR compared with normotensive Wistar-

SUBMITTER: Ghosh K 

PROVIDER: S-EPMC10830874 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature