Operator-derived particles and falling bacteria in biosafety cabinets.

ABSTRACT:

Introduction

To ensure the sterility of cell products that cannot undergo conventional sterilization processes, it is imperative to establish and maintain a clean room environment, regulated through environmental monitoring, including particle counts. Nevertheless, the impact of particles generated by operators as potential contaminants remains uncertain. Thus, in this study, we conducted an accelerated test to assess the correlation between particles generated by operators and airborne bacteria, utilizing biosafety cabinets within a typical laboratory setting. These biosafety cabinets create a controlled environment with air conditioning and high-efficiency particulate air (HEPA) filters, offering fundamental data relevant to cell production.

Materials and methods

We conduc

SUBMITTER: Mizuno M 

PROVIDER: S-EPMC10831277 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature