Sulforaphane activates CD8⁺ T cells antitumor response through IL-12RB2/MMP3/FasL-induced MDSCs apoptosis'.

ABSTRACT:

Background

Extensive attention has been given to the role of myeloid-derived suppressor cells (MDSCs) in driving tumor progression and treatment failure. Preclinical studies have identified multiple agents that eliminate MDSCs. However, none have been authorized in the cliniccal ues due to the safety reasons. In the present study, we investigated the efficacy and mechanism of sulforaphane (SFN) to eliminate MDSCs in the tumor microenvironment (TME).

Methods

We monitored SFN effect on tumor growth and the percents or apoptosis of immune cell subsets in mice models bearing LLC or B16 cells. Flow cytometry, quantitative reverse transcription-PCR, immunohistochemistry, ELISA, immunofluorescence, imaging flow cytometry and western blot were performed to validate the role of SFN o

SUBMITTER: Liu J 

PROVIDER: S-EPMC10831471 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature