High diagnostic accuracy of quantitative SARS-CoV-2 spike-binding-IgG assay and correlation with in vitro viral neutralizing activity.

ABSTRACT:

Background

Antibody testing can easily evaluate the clinical status of patients, aid in the diagnosis of multisystem inflammatory syndrome, and monitor the immunity level in the population. However, the applicability of serological tests in detecting antibodies against the severe acute respiratory syndrome 2 (SARS-CoV-2) spike-binding protein remains limited. This study aimed to quantify both serum-derived neutralizing immunoglobulin-G (IgG) antibody activity and the amount of anti-SARS-CoV-2 Spike-IgG (S-IgG) in convalescent sera/plasmas and evaluate the direct correlation between the in vitro IgG-EC₅₀ values and S-IgG values.

Methods

We evaluated the neutralizing activity of purified IgG (IgG-EC₅₀), quantified S-IgG in the serum/plas

SUBMITTER: Iwamoto N 

PROVIDER: S-EPMC10831606 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature