Direct observation of cytoskeleton-dependent trafficking of miRNA visualized by the introduction of pre-miRNA.

ABSTRACT: MicroRNA (miRNA) plays physiologically and pathologically important roles in post-transcriptional regulation. Although miRNA has been suggested to dynamically interact with cellular organelles, the dynamicity of intracellular miRNA behavior has remained unclear. Here, by introducing fluorescently labeled pre-miRNA into living cells, we improved the miRNA visualization method using exogenous miRNA precursors. Through the combination of our miRNA visualization method and single-molecule sensitive fluorescence microscopy, we quantitatively analyzed the process of miRNA maturation. Furthermore, single-particle tracking of fluorescent miRNA in cells revealed the directed movements of miRNA on cytoskeletal components (i.e., microtubules and actin filaments). Our results also suggest that cytoske

SUBMITTER: Ishikawa T 

PROVIDER: S-EPMC10831896 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature