Preclinical translational platform of neuroinflammatory disease biology relevant to neurodegenerative disease.

ABSTRACT: Neuroinflammation is a key driver of neurodegenerative disease, however the tools available to model this disease biology at the systems level are lacking. We describe a translational drug discovery platform based on organotypic culture of murine cortical brain slices that recapitulate disease-relevant neuroinflammatory biology. After an acute injury response, the brain slices assume a chronic neuroinflammatory state marked by transcriptomic profiles indicative of activation of microglia and astrocytes and loss of neuronal function. Microglia are necessary for manifestation of this neuroinflammation, as depletion of microglia prior to isolation of the brain slices prevents both activation of astrocytes and robust loss of synaptic function genes. The transcriptomic pattern of neuroinflammat

SUBMITTER: Larson KC 

PROVIDER: S-EPMC10832185 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature