Development of a highly sensitive and specific intact proviral DNA assay for HIV-1 subtype B and C.

ABSTRACT:

Introduction

HIV reservoir quantification is essential for evaluation of HIV curative strategies and may provide valuable insights about reservoir dynamics during antiretroviral therapy. The Intact Proviral DNA Assay (IPDA) provides the unique opportunity to quantify the intact and defective reservoir. The current IPDA is optimized for HIV-1 subtype B, the dominant subtype in resource-rich settings. However, subtype C is dominant in Sub-Saharan Africa, jointly accounting for around 60% of the pandemic. We developed an assay capable of quantifying intact and defective proviral HIV-1 DNA of subtype B and C.

Methods

Primer and probe sequences were strategically positioned at conserved regions in psi and env and adapted to subtype B&C. In silico analysis of 752 subtype B and 697

SUBMITTER: Buchholtz NVEJ 

PROVIDER: S-EPMC10832250 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature