Investigation of exopolysaccharide formation and its impact on anaerobic succinate production with Vibrio natriegens.

ABSTRACT: Vibrio natriegens is an emerging host for biotechnology due to its high growth and substrate consumption rates. In industrial processes typically fed-batch processes are applied to obtain high space-time yields. In this study, we established an aerobic glucose-limited fed-batch fermentation with the wild type (wt) of V. natriegens which yielded biomass concentrations of up to 28.4 g_X  L^-1 . However, we observed that the viscosity of the culture broth increased by a factor of 800 at the end of the cultivation due to the formation of 157 ± 20 mg exopolysaccharides (EPS) L^-1 . Analysis of the genomic repertoire revealed several genes and gene clusters associated with EPS formation. Deletion of the transcriptional regulator cpsR in V. natriegens wt did not redu

SUBMITTER: Schulze C 

PROVIDER: S-EPMC10832516 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature