Exploring engineered vesiculation by Pseudomonas putida KT2440 for natural product biosynthesis.

ABSTRACT: Pseudomonas species have become promising cell factories for the production of natural products due to their inherent robustness. Although these bacteria have naturally evolved strategies to cope with different kinds of stress, many biotechnological applications benefit from engineering of optimised chassis strains with specially adapted tolerance traits. Here, we explored the formation of outer membrane vesicles (OMV) of Pseudomonas putida KT2440. We found OMV production to correlate with the recombinant production of a natural compound with versatile beneficial properties, the tripyrrole prodigiosin. Further, several P. putida genes were identified, whose up- or down-regulated expression allowed controlling OMV formation. Finally, genetically triggering vesiculation in production strains

SUBMITTER: Bitzenhofer NL 

PROVIDER: S-EPMC10832525 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature