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Anaerobic glucose uptake in Pseudomonas putida KT2440 in a bioelectrochemical system.


ABSTRACT: Providing an anodic potential in a bio-electrochemical system to the obligate aerobe Pseudomonas putida enables anaerobic survival and allows the cells to overcome redox imbalances. In this setup, the bacteria could be exploited to produce chemicals via oxidative pathways at high yield. However, the absence of anaerobic growth and low carbon turnover rates remain as obstacles for the application of such an electro-fermentation technology. Growth and carbon turnover start with carbon uptake into the periplasm and cytosol. P. putida KT2440 has three native transporting systems for glucose, each differing in energy and redox demand. This architecture previously led to the hypothesis that internal redox and energy constraints ultimately limit cytoplasmic carbon utilization in a bio-electrochem

SUBMITTER: Pause L 

PROVIDER: S-EPMC10832537 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature