Characterising the metabolic rewiring of extremely slow growing Komagataella phaffii.

ABSTRACT: Retentostat cultivations have enabled investigations into substrate-limited near-zero growth for a number of microbes. Quantitative physiology at these near-zero growth conditions has been widely discussed, yet characterisation of the fluxome is relatively under-reported. We investigated the rewiring of metabolism in the transition of a recombinant protein-producing strain of Komagataella phaffii to glucose-limited near-zero growth rates. We used cultivation data from a 200-fold range of growth rates and comprehensive biomass composition data to integrate growth rate dependent biomass equations, generated using a number of different approaches, into a K. phaffii genome-scale metabolic model. Here, we show that a non-growth-associated maintenance value of 0.65  mmol ATP g CDW - 1 h - 1

SUBMITTER: Coltman BL 

PROVIDER: S-EPMC10832545 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature