Genetically engineered CD80-pMHC-harboring extracellular vesicles for antigen-specific CD4⁺ T-cell engagement.

ABSTRACT: The identification of low-frequency antigen-specific CD4⁺ T cells is crucial for effective immunomonitoring across various diseases. However, this task still encounters experimental challenges necessitating the implementation of enrichment procedures. While existing antigen-specific expansion technologies predominantly concentrate on the enrichment of CD8⁺ T cells, advancements in methods targeting CD4⁺ T cells have been limited. In this study, we report a technique that harnesses antigen-presenting extracellular vesicles (EVs) for stimulation and expansion of antigen-specific CD4⁺ T cells. EVs are derived from a genetically modified HeLa cell line designed to emulate professional antigen-presenting cells (APCs) by expressing key costimulatory mo

SUBMITTER: Ishina IA 

PROVIDER: S-EPMC10833362 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature