C-SPAM: an open-source time-resolved specimen vitrification device with light-activated molecules.
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ABSTRACT:
SUBMITTER: Montano Romero A
PROVIDER: S-EPMC10833387 | biostudies-literature | 2024 Jan
REPOSITORIES: biostudies-literature
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C-SPAM: an open-source time-resolved specimen vitrification device with light-activated molecules.
Montaño Romero Alejandra A Bonin Calli C Twomey Edward C EC
IUCrJ 20240101 Pt 1
Molecular structures can be determined in vitro and in situ with cryo-electron microscopy (cryo-EM). Specimen preparation is a major obstacle in cryo-EM. Typical sample preparation is orders of magnitude slower than biological processes. Time-resolved cryo-EM (TR-cryo-EM) can capture short-lived states. Here, Cryo-EM sample preparation with light-activated molecules (C-SPAM) is presented, an open-source, photochemistry-coupled device for TR-cryo-EM that enables millisecond resolution and tunable ...[more]
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