Comparison of permeable cell culture inserts for use in culture of a human in vitro air-liquid interface model system.

ABSTRACT: In this study, we compared 12 mm cell culture inserts with permeable polyester membranes (0.4 μm pores) from two different manufacturers: CELLTREAT® and Corning®. Physical dimensions and masses of the inserts were found to be very similar between the two brands, with CELLTREAT® inserts having a slightly smaller diameter and growth area (11.91 mm; 1.11 cm² ) compared to Corning® Transwells® (12 mm; 1.13 cm² ). We compared cell differentiation outcomes of human nasal epithelial cells (HNECs) at air-liquid interface grown on inserts from the two different manufacturers, including trans-epithelial electrical resistance, ciliary beat frequency, ciliated area, and gene expression. HNECs from three male donors were used for all endpoints. No statistically significant differe

SUBMITTER: Brocke SA 

PROVIDER: S-EPMC10834316 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature