Lack of vitamin D signalling shifts skeletal muscles towards oxidative metabolism.

ABSTRACT:

Background

Mice lacking vitamin D receptor (VDR) exhibit a glycogen storage disorder, disrupting carbohydrate utilization in muscle. Here, we asked if the defective carbohydrate metabolism alters the fat utilization by the skeletal muscles of vdr^-/- mice.

Methods

To check the effect of high-fat-containing diets on muscle mass and metabolism of vdr^-/- mice, we subjected them to two different milk fat-based diets (milk fat diet with 60% of energy from milk fat and milk-based diet [MBD] with 37% of energy from milk fat) and lard-based high-fat diet (HFD) containing 60% of energy from lard fat. Skeletal muscles and pancreas from these mice were analysed using RNA sequencing, quantitative reverse transcription polymerase chain reaction and western blot to un

SUBMITTER: Das A 

PROVIDER: S-EPMC10834326 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature