Engineering of Bacillus thuringiensis Cry2Ab toxin for improved insecticidal activity.

ABSTRACT: Bacillus thuringiensis Cry2Ab toxin was a widely used bioinsecticide to control lepidopteran pests all over the world. In the present study, engineering of Bacillus thuringiensis Cry2Ab toxin was performed for improved insecticidal activity using site-specific saturation mutation. Variants L183I were screened with lower LC₅₀ (0.129 µg/cm²) against P. xylostella when compared to wild-type Cry2Ab (0.267 µg/cm²). To investigate the molecular mechanism behind the enhanced activity of variant L183I, the activation, oligomerization and pore-formation activities of L183I were evaluated, using wild-type Cry2Ab as a control. The results demonstrated that the proteolytic activation of L183I was the same as that of wild-type Cry2Ab. However, variant L183I displayed hi

SUBMITTER: Fu BW 

PROVIDER: S-EPMC10834393 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature