Investigating the effects of PTEN mutations on cGAS-STING pathway in glioblastoma tumours.

ABSTRACT:

Background

PTEN is a tumour suppressor gene and well-known for being frequently mutated in several cancer types. Loss of immunogenicity can also be attributed to PTEN loss, because of its role in establishing the tumour microenvironment. Therefore, this study aimed to represent the link between PTEN and cGAS-STING activity, a key mediator of inflammation, in tumour samples of glioblastoma patients.

Methods

Tumour samples of 36 glioblastoma patients were collected. After DNA isolation, all coding regions of PTEN were sequenced and analysed. PTEN expression status was also evaluated by qRT-PCR, western blot, and immunohistochemical methods. Interferon-stimulated gene expressions, cGAMP activity, CD8 infiltration, and Granzyme B expression levels were determined especially for

SUBMITTER: Dogan E 

PROVIDER: S-EPMC10834568 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature