Structural effects of inosine substitution in telomeric DNA quadruplex.

ABSTRACT: The telomeric DNA, a distal region of eukaryotic chromosome containing guanine-rich repetitive sequence of (TTAGGG)n, has been shown to adopt higher-order structures, specifically G-quadruplexes (G4s). Previous studies have demonstrated the implication of G4 in tumor inhibition through chromosome maintenance and manipulation of oncogene expression featuring their G-rich promoter regions. Besides higher order structures, several regulatory roles are attributed to DNA epigenetic markers. In this work, we investigated how the structural dynamics of a G-quadruplex, formed by the telomeric sequence, is affected by inosine, a prevalent modified nucleotide. We used the standard (TTAGGG)_n telomere repeats with guanosine mutated to inosine at each G position. Sequences (GGG)₄,

SUBMITTER: Zheng YY 

PROVIDER: S-EPMC10834636 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature