Functional and structural analysis of a cyclization domain in a cyclic β-1,2-glucan synthase.

ABSTRACT: Cyclic β-1,2-glucan synthase (CGS) is a key enzyme in production of cyclic β-1,2-glucans (CβGs) which are involved in bacterial infection or symbiosis to host organisms. Nevertheless, a mechanism of cyclization, the final step in the CGS reaction, has not been fully understood. Here we performed functional and structural analyses of the cyclization domain of CGS alone from Thermoanaerobacter italicus (TiCGS_Cy). We first found that β-glucosidase-resistant compounds are produced by TiCGS_Cy with linear β-1,2-glucans as substrates. The ¹H-NMR analysis revealed that these products are CβGs. Next, action pattern analyses using β-1,2-glucooligosaccharides revealed a unique reaction pattern: exclusive transglycosylation without hydrolysis and a hexasaccharide being

SUBMITTER: Tanaka N 

PROVIDER: S-EPMC10834661 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature