Targeted m⁶A demethylation of ITGA6 mRNA by a multisite dCasRx-m⁶A editor inhibits bladder cancer development.

ABSTRACT:

Introduction

N6-methyladenosine (m⁶A) modification contributes to the pathogenesis and development of various cancers, including bladder cancer (BCa). In particular, integrin α6 (ITGA6) promotes BCa progression by cooperatively regulating multisite m⁶A modification. However, the therapeutic effect of targeting ITGA6 multisite m⁶A modifications in BCa remains unknown.

Objectives

We aim to develop a multisite dCasRx- m⁶A editor for assessing the effects of the multisite dCasRx-m⁶A editor targeted m⁶A demethylation of ITGA6 mRNA in BC growth and progression.

Methods

The multisite dCasRx- m⁶A editor was generated by cloning. m⁶A-methylated RNA immunoprecipitation (meRIP), luciferase re

SUBMITTER: Ying X 

PROVIDER: S-EPMC10834799 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature