Structural analyses of β₂-glycoprotein I: is there a circular conformation?

ABSTRACT:

Background

Antiphospholipid antibodies targeting β₂-glycoprotein I (β₂GPI) cause thrombosis and pregnancy morbidity in antiphospholipid syndrome (APS) patients. How these antibodies recognize β₂GPI remains controversial.

Objectives

This study aimed to elucidate the structure of β₂GPI and evaluate how pathogenic anti-domain I (DI) antibodies recognize it in human plasma.

Methods

β₂GPI was made recombinant and purified from human plasma using different protocols. Structural and functional analyses were conducted using orthogonal techniques, namely, electron microscopy, size-exclusion chromatography, single-molecule Förster resonance energy transfer, and microfluidic diffusional sizing.

Results

Electron micros

SUBMITTER: Kumar S 

PROVIDER: S-EPMC10834832 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature