Improvements for recording retinal function with Microelectrode Arrays.

ABSTRACT: A microelectrode array (MEA) is a configuration of multiple electrodes that enables the concurrent targeting of multiple sites for extracellular recording and stimulation. By utilizing light pulses or electrical stimulations, MEA recordings unveil the complex patterns of electrical activities that arise from the signaling processes within the retinal network. Here, we present a stepwise approach for using microelectrode arrays (MEAs) for recording action potentials from the mouse retina in response to electrical and light stimuli. We provide detailed techniques accompanied by description of a custom optical system, example recordings, troubleshooting guidelines, and data processing methods including spike sorting and code resources for analyzing light and electrical responses. The comprehe

SUBMITTER: Rathbun DL 

PROVIDER: S-EPMC10834997 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature