Interactions of ferulic acid and ferulic acid methyl ester with endogenous proteins: Determination using the multi-methods.

ABSTRACT: Ferulic acid (FA) and ferulic acid methyl ester (FAM) are important phenolic compounds in Baijiu. In this study, the interaction of FA and FAM with human serum albumin (HSA) and lysozyme (LZM) was investigated using multispectral methods and molecular dynamics simulation. FA and FAM could interact with HSA and LZM, changing the conformation and hydrophilicity of the protein. The quenching mechanisms of FA-HSA, FA-LZM, FAM-HSA, and FAM-LZM were all static-quenching. In the FA-HSA, FAM-HSA, and FA-LZM systems, the interaction forces were mainly hydrophobic interactions and hydrogen bonding. In the FAM-LZM system, the interaction forces were mainly hydrophobic interactions, hydrogen bonding, and van der Waals force. Common metal ions such as K⁺, Ca²⁺, Cu²⁺, Mg

SUBMITTER: Yang Y 

PROVIDER: S-EPMC10835327 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature