Efficient production of 2'-fucosyllactose from fructose through metabolically engineered recombinant Escherichia coli.

ABSTRACT:

Background

The biosynthesis of human milk oligosaccharides (HMOs) using several microbial systems has garnered considerable interest for their value in pharmaceutics and food industries. 2'-Fucosyllactose (2'-FL), the most abundant oligosaccharide in HMOs, is usually produced using chemical synthesis with a complex and toxic process. Recombinant E. coli strains have been constructed by metabolic engineering strategies to produce 2'-FL, but the low stoichiometric yields (2'-FL/glucose or glycerol) are still far from meeting the requirements of industrial production. The sufficient carbon flux for 2'-FL biosynthesis is a major challenge. As such, it is of great significance for the construction of recombinant strains with a high stoichiometric yield.

Results

In the present stu

SUBMITTER: You R 

PROVIDER: S-EPMC10835893 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature