Deep orange gene editing triggers temperature-sensitive lethal phenotypes in Ceratitis capitata.

ABSTRACT:

Background

The Mediterranean fruit fly, Ceratitis capitata, is a significant agricultural pest managed through area-wide integrated pest management (AW-IPM) including a sterile insect technique (SIT) component. Male-only releases increase the efficiency and cost-effectiveness of SIT programs, which can be achieved through the development of genetic sexing strains (GSS). The most successful GSS developed to date is the C. capitata VIENNA 8 GSS, constructed using classical genetic approaches and an irradiation-induced translocation with two selectable markers: the white pupae (wp) and temperature-sensitive lethal (tsl) genes. However, currently used methods for selecting suitable markers and inducing translocations are stochastic and non-specific, resulting in a laborious and time-co

SUBMITTER: Sollazzo G 

PROVIDER: S-EPMC10835909 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature