Extracellular CIRP induces CD4CD8αα intraepithelial lymphocyte cytotoxicity in sepsis.

ABSTRACT:

Background

In sepsis, intestinal barrier dysfunction is often caused by the uncontrolled death of intestinal epithelial cells (IECs). CD4CD8αα intraepithelial lymphocytes (IELs), a subtype of CD4⁺ T cells residing within the intestinal epithelium, exert cytotoxicity by producing granzyme B (GrB) and perforin (Prf). Extracellular cold-inducible RNA-binding protein (eCIRP) is a recently identified alarmin which stimulates TLR4 on immune cells to induce proinflammatory responses. Here, we hypothesized that eCIRP enhances CD4CD8αα IEL cytotoxicity and induces IEC death in sepsis.

Methods

We subjected wild-type (WT) and CIRP^-/- mice to sepsis by cecal ligation and puncture (CLP) and collected the small intestines to isolate IELs. The expression of GrB and Pr

SUBMITTER: Akama Y 

PROVIDER: S-EPMC10835974 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature