The comparison of adipose-derived stromal cells (ADSCs) delivery method in a murine model of hindlimb ischemia.

ABSTRACT:

Background

Adipose-derived stromal cells (ADSCs) demonstrate ability to promote tissue healing and down-regulate excessive inflammation. ADSCs have been used to treat critical limb ischemia in preclinical and clinical trials, but still, there is little known about their optimal delivery strategy. To date, no direct analysis of different methods of ADSCs delivery has been performed in the hindlimb ischemia model. Therefore, in this study we focused on the therapeutic efficacy of different ADSCs delivery methods in a murine model of hindlimb ischemia.

Methods

For the hADSCs isolation, we used the subcutaneous adipose tissue collected during the surgery. The murine hindlimb ischemia was used as a model. The unilateral femoral artery ligation was performed on 10-12-week-old male

SUBMITTER: Pilny E 

PROVIDER: S-EPMC10836003 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature