Discrimination of pancreato-biliary cancer and pancreatitis patients by non-invasive liquid biopsy.

ABSTRACT:

Background

Current diagnostics for the detection of pancreato-biliary cancers (PBCs) need to be optimized. We therefore propose that methylated cell-free DNA (cfDNA) derived from non-invasive liquid biopsies serves as a novel biomarker with the ability to discriminate pancreato-biliary cancers from non-cancer pancreatitis patients.

Methods

Differentially methylated regions (DMRs) from plasma cfDNA between PBCs, pancreatitis and clinical control samples conditions were identified by next-generation sequencing after enrichment using methyl-binding domains and database searches to generate a discriminatory panel for a hybridization and capture assay with subsequent targeted high throughput sequencing.

Results

The hybridization and capture panel, covering around 74 kb in

SUBMITTER: Hartwig C 

PROVIDER: S-EPMC10836044 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature