Extracellular Proximity Labeling Reveals an Expanded Interactome for the Matrisome Protein TIMP2.

ABSTRACT: Classical methods of investigating protein-protein interactions (PPIs) are generally performed in non-living systems, yet in recent years new technologies utilizing proximity labeling (PL) have given researchers the tools to explore proximal PPIs in living systems. PL has distinct advantages over traditional protein interactome studies, such as the ability to identify weak and transient interactions in vitro and in vivo. Most PL studies are performed on targets within the cell or on the cell membrane. We have adapted the original PL method to investigate PPIs within the extracellular compartment, using both BioID2 and TurboID, that we term extracellular PL (ePL). To demonstrate the utility of this modified technique, we investigate the interactome of the widely expressed matrisome protein

SUBMITTER: Peeney D 

PROVIDER: S-EPMC10836090 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature