Novel insights into the isolation of extracellular vesicles by anion exchange chromatography.

ABSTRACT: Extracellular vesicles (EVs) are membrane structures enclosed by a lipid bilayer that are released into the extracellular space by all types of cells. EVs are involved in many physiological processes by transporting biologically active substances. Interest in EVs for diagnostic biomarker research and therapeutic drug delivery applications has increased in recent years. The realization of the full therapeutic potential of EVs is currently hampered by the lack of a suitable technology for the isolation and purification of EVs for downstream pharmaceutical applications. Anion Exchange Chromatography (AEX) is an established method in which specific charges on the AEX matrix can exploit charges on the surface of EVs and their interactions to provide a productive and scalable separation and puri

SUBMITTER: Koch LF 

PROVIDER: S-EPMC10836363 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature