Ontology highlight
ABSTRACT:
SUBMITTER: Wang T
PROVIDER: S-EPMC10837120 | biostudies-literature | 2024 Feb
REPOSITORIES: biostudies-literature
Wang Tiantian T Shen Wanjun W Li Lin L Wang Haoran H Zhang Min M Chen Xiangmei X
Scientific reports 20240202 1
Preparation of kidney tissue single-cell suspensions is the basis of single-cell sequencing, flow cytometry and primary cell culture, but it is difficult to prepare high quality whole kidney single-cell suspensions because of the complex structure of the kidney. We explored a technique called stepwise enzymatic digestion (StE) method for preparing a single-cell suspension of rat whole kidney tissue which contained three main steps. The first step is to cut the kidney into a homogenate. The secon ...[more]